Technology offer : Production of recombinant proteins in monocotyledons cells

Technology's description

This technique allows the production of recombinant proteins in monocotyledon vegetal cells.

The sequence coding for the protein of interest is inserted within a DNA construction integrating in the mean time a viral vector (Rice yellow mottle virus) which will amplify the replication, it is called the amplicon technology.

These DNA constructions, containing both sequences for the recombinant protein and the viral vector, are introduced in plant cells by transient transformation, i.e.  via a  vector.

Once introduced in the vegetal cells, the amplicon will be traduced in viral RNA that will be replicated and allow an amplified production of the protein.

The gene of interest is not integrated in the genome of the plant: the plants are not GMO.



Advantages

  • Vegetal bioreactors are particularly interesting for their low cost and the facility to implement them compared to other bioproduction systems, based on yeast, bacteria or mammals cells.
  • The absence of contamination risk by an animal pathogen is of major interest in the pharmaceutical context.
  • Transient transformation makes it possible to transfer at will and effectively DNA constructions coding protein of interest simply by infiltration in the leaves, when stable transformation is more complicated and longer to implement.
  • Currently, there is no amplicon tool available for monocotyledons.
Ref : OT-00039

Area of activity : AGRONOMY

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Industrial applications

  • Molecular pharming, biosynthesis of high interest compounds

Intellectual property

  • Patented technology

Technology transfer

  • Co-development with licensing agreement
  • Know-how
  • License

CONTACT


Jean-François BIELA
Business developer

jean-francois.biela@cvt-sud.fr
P: +33 (0)4 91 99 94 73