Technology offer : Production of recombinant proteins in monocotyledons cells
This technique allows the production of recombinant proteins in monocotyledon vegetal cells.
The sequence coding for the protein of interest is inserted within a DNA construction integrating in the mean time a viral vector (Rice yellow mottle virus) which will amplify the replication, it is called the amplicon technology.
These DNA constructions, containing both sequences for the recombinant protein and the viral vector, are introduced in plant cells by transient transformation, i.e. via a vector.
Once introduced in the vegetal cells, the amplicon will be traduced in viral RNA that will be replicated and allow an amplified production of the protein.
The gene of interest is not integrated in the genome of the plant: the plants are not GMO.
Ref : OT-00039
Area of activity : AGRONOMY
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